Surgically resected esophageal cancer tissue was stained using anti-KIAA 0513 antibody (rabbit polyclonal antibodies, Atlas Antibodies), followed by immunohistochemical staining using DAB substrate (Abcam). Photomicrographs were obtained using a light microscope (BA210E, Shimazu) at x100 magnification.
See details in Hiwasa, et. al. (2024) Med Int (Lond).
Takaki Hiwasa, Yoichi Yoshida, Masaaki Kubota, Shu-Yang Li, Bo-Shi Zhang, Tomoo Matsutani, Seiichiro Mine, Toshio Machida, Masaaki Ito, Satoshi Yajima, Mikako Shirouzu, Shigeyuki Yokoyama, Mizuki Sata, Kazumasa Yamagishi, Hiroyasu Iso, Norie Sawada, Shoichiro Tsugane, Minoru Takemoto, Aiko Hayashi, Koutaro Yokote, Yoshio Kobayashi, Kazuyuki Matsushita, Koichiro Tatsumi, Hirotaka Takizawa, Go Tomiyoshi, Hideaki Shimada, Yoshinori Higuchi (2024) Serum anti‑KIAA0513 antibody as a common biomarker for mortal atherosclerotic and cancerous diseases., Medicine international, Volume 4, Number 5, pp. 45
Published in 2024 Sep-Oct (Electronic publication in June 19, 2024, midnight )
(Abstract) Numerous antibody biomarkers have been reported for cancer and atherosclerosis-related diseases. The major complications of atherosclerosis and diabetes mellitus (DM) are acute ischemic stroke (AIS), cardiovascular disease (CVD) and chronic kidney disease (CKD). Cancer development is accompanied by arterial disorders, such as angiogenesis and atherosclerosis, and DM is a risk factor for the development of certain types of cancer. Atherosclerosis-related diseases and cancers are therefore interrelated and could be detected using a common biomarker. In the present study, the initial screening using the protein array method identified KIAA0513 as an antigen recognized by serum IgG antibodies in patients with atherosclerosis. The amplified luminescent proximity homogeneous assay-linked immunosorbent assay revealed significantly higher serum antibody levels against recombinant KIAA0513 protein in patients with AIS, transient ischemic attack (TIA), DM, CVD, obstructive sleep apnea syndrome (OSAS), CKD and solid cancers, such as esophageal, gastric, colon, lung and breast cancers, compared with healthy donors. A receiver operating characteristic (ROC) analysis revealed that the highest areas under the ROC curves of anti-KIAA0513 antibodies were obtained for esophageal cancer, nephrosclerosis-type CKD and DM. Spearman's correlation analysis revealed that serum anti-KIAA0513 antibody levels were associated with maximum intima-media thickness and plaque score, which are indices of atherosclerosis and stenosis. Serum anti-KIAA0513 antibody markers appear to be useful for diagnosing AIS, TIA, DM, CVD, OSAS, CKD and solid cancers, and may reflect common arterial alterations leading to atherosclerotic and cancerous diseases.