The mouse (Mus musculus) is the most standard model organism among mammals, but descriptive studies on its development are limited. This dataset was created to provide a deeper understanding of craniofacial morphogenesis in mice.
The histological sections were prepared from wild-type mice at developmental stages 8.5, 9.5, 10.5, 11.5, 12.5, 13.5, 14.5, and 15.5 dpc (days post-coitum), with a focus on the head region. The samples were fixed using Serra's fixative and stored in 70% ethanol. Paraffin sections were prepared following standard protocols. All sections were sagittal, with a thickness of 6 µm for 8.5 and 9.5 dpc, and 9 µm for 10.5 to 15.5 dpc. Peripheral nerves were stained using immunohistochemistry with anti-acetylated tubulin antibody and visualized with DAB. Subsequent staining included alcian blue, hematoxylin, and eosin, performed according to standard procedures.