Detail of FigS7_NIR_tumor_day0



Project
Title
NIR fluorescence images of breast tumour-bearing mice treated with 0.2mg Kadcyla injected with ICG- EGFP-Annexin for 0 day.
Description
NIR fluorescence images of breast tumour-bearing mice treated with 0.2mg Kadcyla injected with ICG- EGFP-Annexin for 0 day.
Release, Updated
2022-11-23
License
CC BY
Kind
Image data
File Formats
.tif
Data size
5.6 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Organism
Homo sapiens ( NCBI:txid9606 )
Strain(s)
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Cell Line
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Reporter
ICG- EGFP-Annexin

Datatype
-
Molecular Function (MF)
Biological Process (BP)
apoptosis ( GO:0097194 )
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
X scale
264.58 micrometer/pixel
Y scale
264.58 micrometer/pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
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Detector model
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Objective model
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Filter set
-

Summary of Methods
See details in Tsuboi S, et. al. (2020) RSC Adv., 10(63):38244-38250.
Related paper(s)

Setsuko Tsuboi, Takashi Jin (2020) Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo., RSC advances, Volume 10, Number 63, pp. 38244-38250

Published in 2020 Oct 15 (Electronic publication in Oct. 16, 2020, midnight )

(Abstract) Indocyanine green (ICG) labelled recombinant annexin V proteins (ICG-EGFP-Annexin V and ICG-mPlum-Annexin V) were synthesized for dual-colour fluorescence imaging of tumour cell apoptosis in vitro and in vivo. The ICG-labelled fluorescent annexin V proteins showed dual (near-infrared and visible) fluorescence emissions with binding ability to phosphatidylserines on the plasma membranes of apoptotic cells. Although several types of fluorescence labelled annexin V (e.g. FITC-annexin V, Cy3- and Cy5-annexin V) have been reported, there are no dual-colour (near-infrared/visible) emitting apoptosis-detection probes which can be used in vitro and in vivo. In this paper, the utilities of the dual-colour fluorescent annexin V are demonstrated for in vitro and in vivo fluorescence imaging of the apoptosis of human breast tumour cells induced by an antibody-drug conjugate, Kadcyla. The results suggest that the present annexin V probes will be useful to visualize the action of anti-cancer drugs against tumours both at the cellular and whole-body level.

Contact
Takashi Jin , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Developmental Dynamics
Contributors

OMERO Dataset
OMERO Project
Source