Detail of Fig3C_WT_PHPKB-GFP_sGCN-TMR
Project
Title
Images of a wild-type AX2 cell co-expressing PHPKB–GFP and sGCN–TMR stimulated by repetitive pulse stimuli.
Description
Images of a wild-type AX2 cell co-expressing PHPKB–GFP and sGCN–TMR stimulated by repetitive pulse stimuli.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.tif
Data size
6.3 MB
Organism
Dictyostelium discoideum
(
NCBI:txid44689
)
Strain(s)
Dictyostelium discoideum AX2 (NCBI:txid366501)
Cell Line
-
Protein names
PHPKB , sGCN
Protein tags
GFP, TMR
Datatype
-
Molecular Function (MF)
cAMP binding
(
GO:0030552
)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
3.9370 micrometer/pixel
Y scale
3.9370 micrometer/pixel
Z scale
-
T scale
3 second per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Tanabe Y, et. al. (2018) J Cell Sci., 131(23):jcs214775.
Related paper(s)
Yuki Tanabe, Yoichiro Kamimura, Masahiro Ueda (2018) Parallel signaling pathways regulate excitable dynamics differently to mediate pseudopod formation during eukaryotic chemotaxis., Journal of cell science, Volume 131, Number 23
Published in 2018 Dec 5 (Electronic publication in Dec. 5, 2018, midnight )
- doi: 10.1242/jcs.214775
-
pmid: 30404836
(Abstract) In eukaryotic chemotaxis, parallel signaling pathways regulate the spatiotemporal pseudopod dynamics at the leading edge of a motile cell through the characteristic dynamics of an excitable system; however, differences in the excitability and the physiological roles of individual pathways remain to be elucidated. Here, we found that two different pathways, mediated by soluble guanylyl cyclase (sGC) and phosphoinositide 3-kinase (PI3K), caused similar all-or-none responses for sGC localization and phosphatidylinositol 3,4,5-trisphosphate production but with different refractory periods, by undertaking simultaneous observations of the excitable properties of the two pathways in Dictyostelium cells. Owing to the shorter refractory period, sGC signaling responded more frequently to chemoattractants, leading to pseudopod formation with higher frequency. sGC excitability was regulated negatively by its product cGMP and by cGMP-binding protein C (GbpC) through the suppression of F-actin polymerization, providing the underlying delayed negative-feedback mechanism for the cyclical pseudopod formation. These results suggest that parallel pathways respond to environmental cues on different timescales in order to mediate chemotactic motility in a manner based on their intrinsic excitability.(MeSH Terms)
Contact
Masahiro Ueda
, Osaka University
, Graduate School of Frontier Biosciences
, Laboratory for Cell Signaling Dynamics
Contributors
OMERO Dataset
OMERO Project
Source