Detail of FigS7e_oocyte_siRrc1_Prc1
Project
Title
Live imaging of oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) after Prc1 RNAi with expression of siRNA-resistant Prc1.
Description
Live imaging of oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) after Prc1 RNAi with expression of siRNA-resistant Prc1.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.lsm
Data size
3.0 GB
Gene symbols
Prc1
Protein names
Map4, H2B
Protein tags
EGFP, mCherry
Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
meiosis I nuclear membrane disassembly
(
GO:0051079
)
Cellular Component (CC)
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
0.1037832 micrometer/pixel
Y scale
0.1037832 micrometer/pixel
Z scale
4 micrometer/slice
T scale
5 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Yoshida S, et. al. (2020) Nat Commun., 11(1):2652.
Related paper(s)
Shuhei Yoshida, Sui Nishiyama, Lisa Lister, Shu Hashimoto, Tappei Mishina, Aurelien Courtois, Hirohisa Kyogoku, Takaya Abe, Aki Shiraishi, Meenakshi Choudhary, Yoshiharu Nakaoka, Mary Herbert, Tomoya S Kitajima (2020) Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes., Nature communications, Volume 11, Number 1, pp. 2652
Published in 2020 May 27 (Electronic publication in May 27, 2020, midnight )
- doi: 10.1038/s41467-020-16488-y
-
pmid: 32461611
(Abstract) Acentrosomal meiosis in oocytes represents a gametogenic challenge, requiring spindle bipolarization without predefined bipolar cues. While much is known about the structures that promote acentrosomal microtubule nucleation, less is known about the structures that mediate spindle bipolarization in mammalian oocytes. Here, we show that in mouse oocytes, kinetochores are required for spindle bipolarization in meiosis I. This process is promoted by oocyte-specific, microtubule-independent enrichment of the antiparallel microtubule crosslinker Prc1 at kinetochores via the Ndc80 complex. In contrast, in meiosis II, cytoplasm that contains upregulated factors including Prc1 supports kinetochore-independent pathways for spindle bipolarization. The kinetochore-dependent mode of spindle bipolarization is required for meiosis I to prevent chromosome segregation errors. Human oocytes, where spindle bipolarization is reportedly error prone, exhibit no detectable kinetochore enrichment of Prc1. This study reveals an oocyte-specific function of kinetochores in acentrosomal spindle bipolarization in mice, and provides insights into the error-prone nature of human oocytes.(MeSH Terms)
- Animals
- Cell Cycle Proteins/metabolism
- Chromosome Segregation
- Cytoskeletal Proteins/metabolism
- Female
- Gametogenesis/physiology
- Kinetochores/metabolism[major]
- Meiosis/physiology
- Mice
- Microtubule-Associated Proteins/metabolism[major]
- Microtubules/metabolism
- Nuclear Proteins/metabolism
- Oocytes/metabolism[major]
- Spindle Apparatus/metabolism[major]
Contact
Tomoya S. Kitajima
, RIKEN
, Center for Biosystems Dynamics Research
, Laboratory for Chromosome Segregation
Contributors
OMERO Dataset
OMERO Project
Source