Detail of Figure1B_EKAREV-NLS_urothelium_FRET
Project
Title
Time-lapse Forster resonance energy transfer (FRET) images of the mouse bladder wall expressing EKAREV-nuclear localization signal (EKAREV-NLS)
Description
Time-lapse Forster resonance energy transfer (FRET) images of the mouse bladder wall expressing EKAREV-nuclear localization signal (EKAREV-NLS)
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
Data size
31.0 MB
Datatype
-
Molecular Function (MF)
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Biological Process (BP)
activation of MAPERK kinase
(
GO:0000186
)
cell migration
(
GO:0016477
)
Cellular Component (CC)
nucleus
(
GO:0005634
)
Biological Imaging Method
time lapse microscopy
(
Fbbi:00000249
)
X scale
0.414 micrometer/pixel
Y scale
0.414 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
See details in Sano T, et. al. (2018) The American J. of Pathol., 188(11): 2564-2573.
Related paper(s)
Takeshi Sano, Takashi Kobayashi, Osamu Ogawa, Michiyuki Matsuda (2018) Gliding Basal Cell Migration of the Urothelium during Wound Healing., The American journal of pathology, Volume 188, Number 11, pp. 2564-2573
Published in 2018 Nov (Electronic publication in Aug. 16, 2018, midnight )
- doi: 10.1016/j.ajpath.2018.07.010
-
pmid: 30121259
(Abstract) Collective cell migration during wound healing has been extensively studied in the epidermis. However, it remains unknown whether the urothelium repairs wounds in a manner similar to the epidermis. By in vivo two-photon excitation microscopy of transgenic mice that express fluorescent biosensors, we studied the collective cell migration of the urothelium in comparison with that of the epidermis. In vivo time-lapse imaging revealed that, even in the absence of a wound, urothelial cells continuously moved and sometimes glided as a sheet over the underlying lamina propria. On abrasion of the epithelium, the migration speed of each epidermal cell was inversely correlated with the distance to the wound edge. Repetitive activation waves of extracellular signal-regulated kinase (ERK) were generated at and propagated away from the wound edge. In contrast, urothelial cells glided as a sheet over the lamina propria without any ERK activation waves. Accordingly, the mitogen-activated protein kinase/ERK kinase inhibitor PD0325901 decreased the migration velocity of the epidermis but not the urothelium. Interestingly, the tyrosine kinase inhibitor dasatinib inhibited migration of the urothelium as well as the epidermis, suggesting that the gliding migration of the urothelium is an active, not a passive, migration. In conclusion, the urothelium glides over the lamina propria to fill wounds in an ERK-independent manner, whereas the epidermis crawls to cover wounds in an ERK-dependent manner.(MeSH Terms)
- Animals
- Cell Movement/physiology[major]
- Cell Proliferation
- Cells, Cultured
- Epidermis/physiology[major]
- Extracellular Signal-Regulated MAP Kinases/metabolism
- Female
- Intravital Microscopy
- MAP Kinase Signaling System/physiology
- Mice
- Mice, Transgenic
- Time-Lapse Imaging
- Urothelium/cytology
- Urothelium/physiology[major]
- Wound Healing/physiology[major]
Contact
Michiyuki Matsuda
, Kyoto University
, Graduate School of Biostudies
, Laboratory of Bioimaging and Cell Signaling
Contributors
OMERO Dataset
OMERO Project
Source