Detail of fig7_RSAdetection_4w
Project
Title
Immunofluorescence image of 46a IPS-RPE cells incubaed with the serum from the TLHM6 monkey for 4 weeks
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
651.2 KB
Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
retinal pigment epithelium development
(
GO:0003406
)
Cellular Component (CC)
cell
(
GO:0005623
)
Biological Imaging Method
XYZ Scale
XY: 1.2503 micrometer/pixel, Z: NA
T scale
-
Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
ZEISS LSM700
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Sugita et al. (2017) Stem Cell Reports, 9(5): 1501-1515.
Related paper(s)
Sunao Sugita, Kenichi Makabe, Shota Fujii, Yuko Iwasaki, Hiroyuki Kamao, Takashi Shiina, Kazumasa Ogasawara, Masayo Takahashi (2017) Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models., Stem cell reports, Volume 9, Number 5, pp. 1501-1515
Published in 2017 Nov 14 (Electronic publication in Nov. 5, 2017, midnight )
- doi: 10.1016/j.stemcr.2017.10.003
-
pmid: 29103970
(Abstract) Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluripotent stem cell (iPSC)-RPE allografts and determined whether RPE-specific antibody could be detected in these models. We detected alloantibodies in the serum from recipient monkeys that had immune attacks in the retina in an immunofluorescent assay using the transplanted iPSC-RPE cells as the antigen. In addition to T cell and antigen-presenting cell immunity, peripheral blood cells and lymph nodes in animal models with allogeneic iPSC-RPE cells also had activated B cells, which were probably secreting alloantibodies. Using serum and transplanted cells, alloreactive antibody can be detected for the diagnosis of immune rejection after transplantation.(MeSH Terms)
- Animals
- Antigen Presentation
- B-Lymphocytes/immunology
- Cell Differentiation
- Cells, Cultured
- Graft Rejection/immunology[major]
- Graft Rejection/prevention & control
- Induced Pluripotent Stem Cells/cytology
- Induced Pluripotent Stem Cells/immunology
- Induced Pluripotent Stem Cells/transplantation[major]
- Isoantibodies/immunology[major]
- Isoantibodies/therapeutic use
- Macaca fascicularis
- Retinal Pigment Epithelium/cytology
- Retinal Pigment Epithelium/immunology
- Retinal Pigment Epithelium/transplantation[major]
- Stem Cell Transplantation/adverse effects
- Stem Cell Transplantation/methods[major]
- T-Lymphocytes/immunology
- Transplantation, Homologous
Contact
Sunao Sugita
, RIKEN
, Center for Biosystems Dynamics Research
, Laboratory for Retinal Regeneration
Contributors
Sunao Sugita, Kenichi Makabe, Shota Fujii, Yuko Iwasaki, Hiroyuki Kamao, Takashi Shiina, Kazumasa Ogasawara, Masayo Takahashi
OMERO Dataset
OMERO Project
Source