SSBD - SSBD:database - Fig6-S6_Bz-Val-mixture_Mouse1

Detail of Fig6-S6_Bz-Val-mixture_Mouse1_17

SSBD:Repository

Title

Image of concentration response curves for response amplitude and oscillation phase shifts in tracheotomized M/T-GCaMP3 mice.

Release, Updated

2019-11-20

Kind

Image data based on Experiment

Organism

M. musculus ( NCBI:txid10090 )

Strain(s)

Cell Line

Protein tags

GCaMP3

Datatype

calcium imaging data

Molecular Function (MF)

Biological Process (BP)

neuron cell to cell adhesion ( GO:0007158 )

Cellular Component (CC)

axon ( GO:0030424 ) dendrite ( GO:0030425 )

Biological Imaging Method

XYZ Scale

XY: 1.988 micrometer/pixel, Z: NA

T scale

0.04 second for each time interval

Image Acquisition

Experiment type

TimeLapse

Microscope type

ConfocalMicroscope

Acquisition mode

LaserScanningConfocalMicroscopy

Contrast method

Fluorescence

Microscope model

Olympus FV1000MPE

Detector model

Objective model

Olympus XLPLN25XWMP

Filter set

Summary of Methods

See details in Iwata et al. (2017) Neuron, 96(5): 1139-1152.

Related paper(s)

Ryo Iwata, Hiroshi Kiyonari, Takeshi Imai (2017) Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb., Neuron, Volume 96, Number 5, pp. 1139-1152.e7

Published in 2017 Dec 6

doi: 10.1016/j.neuron.2017.11.008
pmid: 29216451
url: https://www.cell.com/neuron/fulltext/S0896-6273(17)31036-X

(Abstract) Mitral and tufted (M/T) cells in the olfactory bulb produce rich temporal patterns of activity in response to different odors. However, it remains unknown how these temporal patterns are generated and how they are utilized in olfaction. Here we show that temporal patterning effectively discriminates between the two sensory modalities detected by olfactory sensory neurons (OSNs): odor and airflow-driven mechanical signals. Sniff-induced mechanosensation generates glomerulus-specific oscillatory activity in M/T cells, whose phase was invariant across airflow speed. In contrast, odor stimulation caused phase shifts (phase coding). We also found that odor-evoked phase shifts are concentration invariant and stable across multiple sniff cycles, contrary to the labile nature of rate coding. The loss of oscillatory mechanosensation impaired the precision and stability of phase coding, demonstrating its role in olfaction. We propose that phase, not rate, coding is a robust encoding strategy of odor identity and is ensured by airflow-induced mechanosensation in OSNs.

(MeSH Terms)

Contact

Takeshi Imai , Kyushu University , Graduate School of Medical Sciences , Department of Developmental Neurophysiology

Contributors

Ryo Iwata, Hiroshi Kiyonari, Takeshi Imai