Detail of Fig5A_process
Project
Title
The processed Raman images of dMet and background signals in wild type Drosophila larval fat body
Description
The processed Raman images of deuterated methionine (dMet) and background signals in wild type Drosophila larval fat body. XXX_Met.jpg is dMet image, and XXX_XPM.jpg is background image. XXX_Met.jpg was genarated from 1.tif and 2.tif which was subtracted 0.tif and 3.tif background signals. XXX_XPM.jpg was generated from 0.tif and 3.tif background signals.
Release, Updated
2025-04-10
License
CC BY
Kind
Image data
File Formats
.jpg
Data size
170.5 KB
Datatype
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Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
X scale
80 micrometer
Y scale
80 micrometer
Z scale
-
T scale
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Image Acquisition
Experiment type
-
Microscope type
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Acquisition mode
-
Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Spratt SJ, Mizuguchi T, Akaboshi H, Kosakamoto H, Okada R, Obata F, Ozeki Y. Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering. Front Chem. 2023 Mar 29;11:1141920.
Related paper(s)
Spencer J Spratt, Takaha Mizuguchi, Hikaru Akaboshi, Hina Kosakamoto, Rina Okada, Fumiaki Obata, Yasuyuki Ozeki (2023) Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering., Frontiers in chemistry, Volume 11, pp. 1141920
Published in 2023 (Electronic publication in March 29, 2023, midnight )
- doi: 10.3389/fchem.2023.1141920
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pmid: 37065821
(Abstract) Introduction: Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging. Methods: Here, we used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporated into Drosophila tissues in vivo. Results: Our results demonstrate that SRS can detect a range of previously uncharacterized cell-to-cell differences in d-Met distribution within a tissue at the subcellular level. Discussion: These results demonstrate the potential of SRS microscopy for metabolic imaging of less abundant but important amino acids such as methionine in tissue.
Contact
Yasuyuki Ozeki
, The University of Tokyo
, Department of Electrical Engineering and Information Systems
, Department of Electrical Engineering and Information Systems
Contributors
Spencer J Spratt, Takaha Mizuguchi, Hikaru Akaboshi, Hina Kosakamoto
OMERO Project
Source