Detail of Fig2C_Ca_conditionUNC13_7
Project
Title
Time-lapse calcium imaging of ASEL in conditioned unc-13 mutant C-elegans
Description
Time-lapse calcium imaging of ASEL, a gustatory neuron, in conditioned unc-13 mutant C-elegans. The mutant worm was conditioned to be attracted to glucose beforehand. The autors used YC2.60 as a calcium indicator, and measured fluorescence intensity ratio of YFP to CFP. Left-half are CFP images, and Right half are YFP images. The glucose concentration was switched from 15 to 0 mM at 50 seconds and then returned to 15 mM at 100 seconds.
Release, Updated
2025-04-10
License
CC BY
Kind
Image data
File Formats
tif
Data size
122.3 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to chemical
(
GO:0042221
)
Cellular Component (CC)
Biological Imaging Method
X scale
0.4 micrometer
Y scale
0.4 micrometer
Z scale
-
T scale
0.5 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Tomioka M, Umemura Y, Ueoka Y, Chin R, Katae K, Uchiyama C, Ike Y, Iino Y. Antagonistic regulation of salt and sugar chemotaxis plasticity by a single chemosensory neuron in Caenorhabditis elegans. PLoS Genet. 2023 Sep 5;19(9):e1010637. PLoS Genet. 2023 Sep 5;19(9):e1010637.
Related paper(s)
Masahiro Tomioka, Yusuke Umemura, Yutaro Ueoka, Risshun Chin, Keita Katae, Chihiro Uchiyama, Yasuaki Ike, Yuichi Iino (2023) Antagonistic regulation of salt and sugar chemotaxis plasticity by a single chemosensory neuron in Caenorhabditis elegans., PLoS genetics, Volume 19, Number 9, pp. e1010637
Published in 2023 Sep 5 (Electronic publication in Sept. 5, 2023, midnight )
- doi: 10.1371/journal.pgen.1010637
-
pmid: 37669262
(Abstract) The nematode Caenorhabditis elegans memorizes various external chemicals, such as ions and odorants, during feeding. Here we find that C. elegans is attracted to the monosaccharides glucose and fructose after exposure to these monosaccharides in the presence of food; however, it avoids them without conditioning. The attraction to glucose requires a gustatory neuron called ASEL. ASEL activity increases when glucose concentration decreases. Optogenetic ASEL stimulation promotes forward movements; however, after glucose conditioning, it promotes turning, suggesting that after glucose conditioning, the behavioral output of ASEL activation switches toward glucose. We previously reported that chemotaxis toward sodium ion (Na+), which is sensed by ASEL, increases after Na+ conditioning in the presence of food. Interestingly, glucose conditioning decreases Na+ chemotaxis, and conversely, Na+ conditioning decreases glucose chemotaxis, suggesting the reciprocal inhibition of learned chemotaxis to distinct chemicals. The activation of PKC-1, an nPKC epsilon/eta ortholog, in ASEL promotes glucose chemotaxis and decreases Na+ chemotaxis after glucose conditioning. Furthermore, genetic screening identified ENSA-1, an ortholog of the protein phosphatase inhibitor ARPP-16/19, which functions in parallel with PKC-1 in glucose-induced chemotactic learning toward distinct chemicals. These findings suggest that kinase-phosphatase signaling regulates the balance between learned behaviors based on glucose conditioning in ASEL, which might contribute to migration toward chemical compositions where the animals were previously fed.
Contact
Masahiro Tomioka, Yuichi Iino
, National Institude of Occupational Safety and Health, Japan, The University of Tokyo
, Division of Industrial Toxicology, Department of Biological Sciences, Graduate School of Science
, Division of Industrial Toxicology, Department of Biological Sciences, Graduate School of Science
Contributors
Risshun Chin
OMERO Project
Source