Detail of Figure6A_20191204_HTR6_1_control
Project
SSBD:Repository
Title
Images of Gas-coupled serotonin receptors HTR6 expressing at the cell surface and whole cell of control HeLa cells.
Description
Images of Gas-coupled serotonin receptors HTR6 expressing at the cell surface and whole cell of control HeLa cells.
Release, Updated
2025-02-17
License
CC-BY
Kind
Image data
File Formats
.tif
Data size
27.6 MB
Datatype
-
Molecular Function (MF)
G protein-coupled serotonin receptor activity
(
GO:0004993
)
Biological Process (BP)
G protein-coupled serotonin receptor signaling pathway
(
GO:0098664
)
Cellular Component (CC)
nucleus
(
GO:0005634
)
Biological Imaging Method
time lapse microscopy
(
Fbbi:00000249
)
X scale
0.74 micrometer/pixel
Y scale
0.74 micrometer/pixel
Z scale
-
T scale
60 seconds of time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Tany R, et. al. Biochem J. 2022 Apr 29;479(8):883-900.
Related paper(s)
Ryosuke Tany, Yuhei Goto, Yohei Kondo, Kazuhiro Aoki (2022) Quantitative live-cell imaging of GPCR downstream signaling dynamics., The Biochemical journal, Volume 479, Number 8, pp. 883-900
Published in 2022 Apr 29
- doi: 10.1042/BCJ20220021
-
pmid: 35383830
-
url: https://portlandpress.com/biochemj/article-abstract/479/8/883/231119/Quantitative-live-cell-imaging-of-GPCR-downstream
(Abstract) G-protein-coupled receptors (GPCRs) play an important role in sensing various extracellular stimuli, such as neurotransmitters, hormones, and tastants, and transducing the input information into the cell. While the human genome encodes more than 800 GPCR genes, only four Galpha-proteins (Galphas, Galphai/o, Galphaq/11, and Galpha12/13) are known to couple with GPCRs. It remains unclear how such divergent GPCR information is translated into the downstream G-protein signaling dynamics. To answer this question, we report a live-cell fluorescence imaging system for monitoring GPCR downstream signaling dynamics. Genetically encoded biosensors for cAMP, Ca2+, RhoA, and ERK were selected as markers for GPCR downstream signaling, and were stably expressed in HeLa cells. GPCR was further transiently overexpressed in the cells. As a proof-of-concept, we visualized GPCR signaling dynamics of five dopamine receptors and 12 serotonin receptors, and found heterogeneity between GPCRs and between cells. Even when the same Galpha proteins were known to be coupled, the patterns of dynamics in GPCR downstream signaling, including the signal strength and duration, were substantially distinct among GPCRs. These results suggest the importance of dynamical encoding in GPCR signaling.(MeSH Terms)
Contact
Yuhei Goto, Kazuhiro Aoki
, National Institutes of Natural Sciences, National Institutes of Natural Sciences
, Exploratory Research Center on Life and Living Systems (ExCELLS), Exploratory Research Center on Life and Living Systems (ExCELLS)
, Quantitative Biology Research Group, Quantitative Biology Research Group
Contributors
OMERO Project
Source