Detail of Fig3B_ParaCompress
Project
Title
Time-series images of ERK activity at the tip of an isolated lung epithelium against compression parallel to the distal-proximal axis
Description
Time-series images of ERK activity at the tip of an isolated lung epithelium against compression parallel to the distal-proximal axis. The authors embedded the tissue within the FGF1-containing Matrigel filled inside a polydimethyl- siloxane chamber, and uniaxially compressed it by 33% parallelly to the distal-proximal axis of the lung epithelium. The images of 2 min, 10 min, 20 min and 30 min after applying compression are reposited. The image before compression is also reposited. Channel1; bright field, Channel2; CFP, Channel3; YFP
Release, Updated
2025-02-13
License
CC BY
Kind
Image data
File Formats
.oib
Data size
56.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
lung lobe formation
signal transduction
Cellular Component (CC)
cytosol
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.53 micrometer
Y scale
0.53 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
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Filter set
-
Summary of Methods
Hirashima T and Matsuda M. ERK-mediated curvature feedback regulates branching morphogenesis in lung epithelial tissue. Curr Biol. 2024 Feb 26;34(4):683-696.e6.
Related paper(s)
Tsuyoshi Hirashima, Michiyuki Matsuda (2024) ERK-mediated curvature feedback regulates branching morphogenesis in lung epithelial tissue., Current biology : CB
Published in 2024 Jan 9 (Electronic publication in Jan. 9, 2024, midnight )
- doi: 10.1016/j.cub.2023.12.049
-
pmid: 38228149
(Abstract) Intricate branching patterns emerge in internal organs due to the recurrent occurrence of simple deformations in epithelial tissues. During murine lung development, epithelial cells in distal tips of the single tube require fibroblast growth factor (FGF) signals emanating from their surrounding mesenchyme to form repetitive tip bifurcations. However, it remains unknown how the cells employ FGF signaling to convert their behaviors to achieve the recursive branching processes. Here, we show a mechano-chemical regulatory system underlying lung branching morphogenesis, orchestrated by extracellular signal-regulated kinase (ERK) as a downstream driver of FGF signaling. We found that tissue-scale curvature regulated ERK activity in the lung epithelium using two-photon live cell imaging and mechanical perturbations. ERK activation occurs specifically in epithelial tissues exhibiting positive curvature, regardless of whether the change in curvature was attributable to morphogenesis or perturbations. Moreover, ERK activation accelerates actin polymerization preferentially at the apical side of cells, mechanically contributing to the extension of the apical membrane, culminating in a reduction of epithelial tissue curvature. These results indicate the existence of a negative feedback loop between tissue curvature and ERK activity that transcends spatial scales. Our mathematical model confirms that this regulatory mechanism is sufficient to generate the recursive branching processes. Taken together, we propose that ERK orchestrates a curvature feedback loop pivotal to the self-organized patterning of tissues.
Contact
Tsuyoshi Hirashima
, National University of Singapore
, Mechanobiology Institute
, Mechanobiology Institute
Contributors
OMERO Project
Source