Detail of Fig5_1h_Lipid
Project
Title
Raman images of lipid in HeLa cells treated with ThioRas
Description
Raman images of lipid in HeLa cells treated with ThioRas (alkyl alphaCNA 1h, a thiol-detecting Raman sensor). The Raman image at at 2853 cm ^{-1}.
Release, Updated
2025-01-31
License
CC BY
Kind
Image data
File Formats
tif
Data size
173.6 KB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
inelastic scattering of photons
(
Fbbi:00000589
)
X scale
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Y scale
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Z scale
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T scale
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Image Acquisition
Experiment type
-
Microscope type
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Acquisition mode
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Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Yamakoshi H, Shibata D, Bando K, Kajimoto S, Kohyama A, Egoshi S, Dodo K, Iwabuchi Y, Sodeoka M, Fujita K, Nakabayashi T. Ratiometric analysis of reversible thia-Michael reactions using nitrile-tagged molecules by Raman microscopy. Chem Commun (Camb). 2023 Dec 7;59(98):14563-14566.
Related paper(s)
Hiroyuki Yamakoshi, Daiki Shibata, Kazuki Bando, Shinji Kajimoto, Aki Kohyama, Syusuke Egoshi, Kosuke Dodo, Yoshiharu Iwabuchi, Mikiko Sodeoka, Katsumasa Fujita, Takakazu Nakabayashi (2023) Ratiometric analysis of reversible thia-Michael reactions using nitrile-tagged molecules by Raman microscopy., Chemical communications (Cambridge, England)
Published in 2023 Nov 21 (Electronic publication in Nov. 21, 2023, midnight )
- doi: 10.1039/d3cc05015g
-
pmid: 37986604
(Abstract) Ratiometric Raman analysis of reversible thia-Michael reactions was achieved using alpha-cyanoacrylic acid (alphaCNA) derivatives. Among alphaCNAs, the smallest derivative, ThioRas (molecular weight: 167 g mol(-1)), and its glutathione adduct were simultaneously detected in various subcellular locations using Raman microscopy.
Contact
Hiroyuki Yamakoshi
, Tohoku University
, Graduate School of Pharmaceutical Sciences
, Graduate School of Pharmaceutical Sciences
Contributors
OMERO Project
Source