Detail of Fig1B_RamanMitoQ
Project
Title
Live-cell Raman images of HeLa cells using a deuterium-incorporated compound, Mito-Q-d15
Description
Time-series Raman images of HeLa cells treated with 2 micromolar Mito-Q-d15 (2251-2322 cm^{-1}), which is deuterated Mito-Q. The images of 10 min and 60 min after Mito-Q-d15 treatment are shown. The mitochondrial uptake of Mito-Q-d15 was clearly observed 10 minutes after treatment. The Raman images of cytochrome c (739-759 cm^{-1}), and their merged images are also reposited.
Release, Updated
2025-01-31
License
CC BY
Kind
Image data
File Formats
tif
Data size
60.9 KB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion
(
GO:0005739
)
Biological Imaging Method
inelastic scattering of photons
(
Fbbi:00000589
)
X scale
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Y scale
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Z scale
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T scale
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Image Acquisition
Experiment type
-
Microscope type
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Acquisition mode
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Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Moriyama S, Mae M, Shibata D, Yamakoshi H, Kajimoto S, Nakabayashi T, Ishimoto T, Mogi K, Sajiki H, Akai S, Sawama Y. Multiple deuteration of triphenylphosphine and live-cell Raman imaging of deuterium-incorporated Mito-Q. Chem Commun (Camb). 2023 Oct 10;59(81):12100-12103.
Related paper(s)
Shogo Moriyama, Miyu Mae, Daiki Shibata, Hiroyuki Yamakoshi, Shinji Kajimoto, Takakazu Nakabayashi, Takayoshi Ishimoto, Kaiki Mogi, Hironao Sajiki, Shuji Akai, Yoshinari Sawama (2023) Multiple deuteration of triphenylphosphine and live-cell Raman imaging of deuterium-incorporated Mito-Q., Chemical communications (Cambridge, England)
Published in 2023 Sep 18 (Electronic publication in Sept. 18, 2023, midnight )
- doi: 10.1039/d3cc04410f
-
pmid: 37721453
(Abstract) All aromatic C-H bonds of triphenylphosphine (PPh(3)) were efficiently replaced by C-D bonds using Ru/C and Ir/C co-catalysts in 2-PrOH and D(2)O, an inexpensive deuterium source. Furthermore, non-radioactive and safe deuterium-incorporated Mito-Q (drug candidate) was prepared from deuterated PPh(3) and used for the live-cell Raman imaging to evaluate the mitochondrial uptake.
Contact
Yoshinari Sawama
, Osaka University
, Graduate School of Pharmaceutical Sciences
, Graduate School of Pharmaceutical Sciences
Contributors
Shogo Moriyama, Daiki Shibata, Hiroyuki Yamakoshi, Shinji Kajimoto, Takakazu Nakabayashi
OMERO Project
Source